Composite
Part:BBa_K2235010:Design
Designed by: Shanlin Tong Group: iGEM17_Stockholm (2017-10-11)
Endo beta galactosidase with T7 promoter, RBS and functional unit
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 246
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The following primers were used to amplify the gblock:
Fwd: CTAGTCTAGAATGTTTGTCTTTATGTTACTATTGCTGCTACCGTTTA
Rev: AAAACTGCAGTTATTCTACAGGAATAAGTTGCCATTGACTTGTCCAATAAGTATTTGGAA
Source
Endo beta galactosidase with T7 promoter, RBS and functional unit